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We are microbiology students of Franciscan University! Our blog is our lab report and we encourage you witness our fabulous adventure of uncovering, and discovering!

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OUR UNKNOWN IS KNOWN

After many many microbiology labs, and tirelessly preforming experiment after experiment, we finally found our bacteria!

Escherichia coli!!!!!! 

Please read our blog to witness the process of uncovering our little bacteria!!!

Immunodetective biokit

In this experiment we were testing immunology and how it applies in testing food purity. First, we had to make four wells in each section of the agar. In section one of the petri dish, we used green dye, red dye, barium chloride, and potassium sulfate. Using the dyes, we were able to see how the agar absorbed the dye. For the second portion of the dish, we used bovine albumin, goat anti-horse albumin, goat anti-bovine albumin, and goat anti-swine albumin. Also in the third, we used all of the solutions, except for the bovine albumin and replaced it with hamburger extract.

ELISA

This test helps detect antibodies in the blood to determine if someone has been exposed to a disease. ELISA is used for diverse purposes such as pregnancy tests, disease detection, detecting illegal drug use, testing indoor air quality, and determining of food is labeled properly. In this experiment, we used purified antigen, positive control, negative control, secondary control, and a substrate into a 12-well strip. We put each fluid into each well individually then waited 5 minutes to allow the antibodies the opportunity to bind to the antigen. After doing this, we would wash out the wells then go onto the next fluid. After putting in the last substrate, we waited for the results; if it turned blue then it was positive for the AIDs antigen, which our substrate was.

UV pen

In order to show us how the Uv pen worked, we took samples of all of the class's unknown bacteria and put it into a beaker of water. The shorter wave lengths of the UV pen kill bacteria!

Ahh..the wonders of a steri-pen. Our professor said that he takes his steri-pen when he travels abroad to different countries. The pen only works if there nothing causing a barrier between the bacteria and the light. For instance, the UV rays cannot pass through something as thin as paper.

Unfortunately, no one would drink the water:(

UV light

We put our bacteria on a streak plate. Covering half of the plate with an index card was supposed to stop the ultraviolet light from killing the bacteria. We put the plate under the UV light for 30 seconds in order to kill the half of the plate that was exposed. 48 hours later, we noted that the bacteria still continued to grow throughout the plate.

Yogurt

Today was a fun day in class!! We made yogurt! 

First, we boiled milk and then proceeded to let it cool until it was 37 degrees C. Next we put the cooled milk into a cup of Kefir and yogurt. Both cups were out in the incubator, and the next class we got to taste and see how much the good bacteria grew. 

To tell the truth, they did not taste good at all:(