ELISA

This test helps detect antibodies in the blood to determine if someone has been exposed to a disease. ELISA is used for diverse purposes such as pregnancy tests, disease detection, detecting illegal drug use, testing indoor air quality, and determining of food is labeled properly. In this experiment, we used purified antigen, positive control, negative control, secondary control, and a substrate into a 12-well strip. We put each fluid into each well individually then waited 5 minutes to allow the antibodies the opportunity to bind to the antigen. After doing this, we would wash out the wells then go onto the next fluid. After putting in the last substrate, we waited for the results; if it turned blue then it was positive for the AIDs antigen, which our substrate was.