The indole test’s purpose was to determine the ability of our bacteria to split amino acids( tryptophan) into indole and pyruvic acid. The simple procedure had us first inoculate the tryptone broth with our bacteria and incubate it for 48 hours at 35 degrees C. After the incubation, we had to add 5 drops of Kovac’s reagent to broth. Right when we added the Kovac solution to the indole tube the top layer turned a redish, pink color which meant that our bacteria was positive for the presence of indole. Our bacteria successfully split the amino acid, tryptophan into indole and pyruvic acid.
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