The citrate test was performed in order to determine if citrate could act as our bacteria’s energy source. Bacteria which use citrate have membrane transporters called Citrate Permease. Citrate Permease convert the citrate to pyruvate and carbon dioxide. For this test we used Simmons citrate agar slant tube. The slant has a green agar that will turn blue if the bacteria utilizes citrate. We inoculated the slant and incubated it for 48 hours. The results turned out to be negative for the utilization of citrate as indicated by the green color of our slant agar.
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