The next experiment detected the oxygen requirements of our unknown. We used thioglycollate broth which reacts with oxygen to form water. A dye present in the broth turns the color pink or blue when oxygen is present, and remains colorless when oxygen is absent. We inoculated our bacteria into the broth and waited 48 hours for the results.
The next lab day we were surprised to find that our bacteria was facultated anaerobic bacteria. Facultated anaerobic bacteria can undergo aerobic respiration and fermentation. It can also grow under aerobic or anaerobic conditions.
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