The purpose of this procedure was to isolate a gram-negative enteric bacilli. The type of agar used is called an Eosin Methylene Blue medium. The medium contains the dyes eosin, methylene, and the sugars lactose, and sucrose. The medium inhibits gram-positive bacteria growth, and only gram-negative bacteria. The primary use of this agar is to identify Enteric bacilli.
We began by inoculating the agar with our bacteria using aseptic technique. We then let the medium incubate for 48 hours. Our results were very specific and exciting!!! The color of our bacterial streak turned to a dark purple, green metallic sheen. Our bacteria is able to ferment lactose and/or sucrose and produce acids. Our lab book stated that our results indicated that our bacteria was Escherichia coli! Exciting! This test helped us in our bacteria identification!!
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