Under a microscope the environmental “stool” sample had two distinct colonies. Our observations consist of the following:
Whole colony form: 1)circular and 2)rhizoid
Colony pigmentation: 1)tan and 2)white/colorless
Appearance of colony surface: both were shiny
Colony Margin: 1)entire and 2)irregular(erose)
Elevation of Colony: both were raised.
We used aseptic technique to transfer one type of colony to a sterile agar broth petri dish called a spread plate. By secluding one colony we could determine its unique characteristics as a pure culture. Carefully, we used flame-sterilized inoculation to transfer one of the colonies to another agar plate. With our inoculating loop we streaked the bacteria onto a streak plate in a quadrant fashion, covering all sides of the petri dish. We then placed the new streak plate into an incubator at 37 degrees C, and waited till the next lab day to record results.
We also inoculated a streak plate with an UNKNOWN bacteria which our teacher gave to us. Our goal throughout our lab classes is to identify the unknown bacteria.
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