Our results for the previous isolation of bacteria shows the distinct colony of our unknown bacteria five days later. The horrific odor seemed to indicate that the bacteria certainly were loving the nutrient agar. The unknown sample seemed to form cilia type filaments.
Today in lab we also transferred our unknown and enviornmental bacteria colonies to a pour plate. In order to begin our transfer, we needed an agar slant tube, and inoculating loop, a brunsen burner, and our original bacteria streak plates. We used aspetic technique once again to insure a complete transfer of the bacteria colonies. First we sterilized the inoculating loop and let it cool. Next we continued to lift the petri plate lid and smear on a small amount of bacteria onto the loop. While holding the inoculating loop, we proceeded to pick up the agar slant tube to take off the lid and sterilize the top before inoculating the agar with the loop in a top to bottom fashion. We then placed the agar slant into an incubator at 37 degrees for about 48 hours in order for the bacteria to grow.
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