Today we attempted three different tests before going to the water plant in Steubenville Ohio. The first test was the Starch Hydrolysis test which tested the bacteria’s ability to digest starches. We inoculated our bacteria onto a starch agar plate and let the bacteria sit for 48 hours in an incubator. After 48 hours we saturated the starch plate with iodine and recorded our results. If a clear color surrounded the bacteria growth it is considered positive for amylase production. Our results showed that there was no indication of amylase production because color appeared around the growth instead of a clear color. When starch reacts with starch it causes a purplish black color to appear. Unfortunately we did not a have a camera available for the results, but just take our word for it. Our bacteria turned out to be negative for amylase production!
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